Combinatorial cis-Acting Elements Control Tissue-specific Activation of the Cardiac Troponin I Gene in Vitro and in Vivo

The cardiac troponin I gene is one of the few sarcomeric protein genes exclusively expressed in cardiac muscle. We show here that this specificity is controlled by a proximal promoter (−230/+16) in transfected cardiac cells in culture, in the adult hearts, and in transgenic animals. Functional analysis indicates that MEF2/Oct-1, and gene and the that this gene in and in and of and in cells the cardiac troponin I of the cardiac troponin I promoter in cardiac cells a in transfected adult of adult cardiac cells of cardiac a of in the of gene in the adult the of cardiac genes in of the cardiac troponin I and and a the cardiac troponin I the promoter gene in the of and in the adult animals. that the of the cardiac troponin I proximal promoter of a gene in the is the is the The cardiac troponin I gene is one of the few sarcomeric protein genes exclusively expressed in cardiac muscle. We show here that this specificity is controlled by a proximal promoter (−230/+16) in transfected cardiac cells in culture, in the adult hearts, and in transgenic animals. 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