Nucleic Acid Selection and the Challenge of Combinatorial Chemistry

Scott E. Osborne; Andrew D. Ellington

doi:10.1021/cr960009c

April 1, 1997Chemical Reviews

Nucleic Acid Selection and the Challenge of Combinatorial Chemistry

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Key Points

To review the principles, methodologies, and synthetic challenges of selecting functional nucleic acids from combinatorial oligonucleotide pools.
Survey of in vitro selection and directed evolution techniques applied to large random-sequence DNA and RNA libraries.
Evaluation of iterative enrichment and amplification cycles for isolating high-affinity binding species and catalytic biopolymers.
In vitro selection enables the isolation of rare functional nucleic acids possessing target affinities comparable to protein antibodies.
Combinatorial nucleic acid libraries overcome traditional rational design limitations to yield novel ribozymes, deoxyribozymes, and ligand-binding aptamers.

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Implication

Review demonstrates the power of nucleic acid selection in molecular discovery, highlighting its capacity to generate custom functional biopolymers.

Abstract

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTNucleic Acid Selection and the Challenge of Combinatorial ChemistryScott E. Osborne and Andrew D. EllingtonView Author Information Department of Chemistry, Indiana University, Bloomington, Indiana 47405 Cite this: Chem. Rev. 1997, 97, 2, 349–370Publication Date (Web):April 1, 1997Publication History Received18 September 1996Revised10 December 1996Published online1 April 1997Published inissue 1 April 1997https://pubs.acs.org/doi/10.1021/cr960009chttps://doi.org/10.1021/cr960009cresearch-articleACS PublicationsCopyright © 1997 American Chemical SocietyRequest reuse permissionsArticle Views2575Altmetric-Citations429LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstract

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Osborne et al. (1997) studied this question.

synapsesocial.com/papers/6a196ad6b71d9c8593890128 https://doi.org/10.1021/cr960009c

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Nucleic Acid Selection and the Challenge of Combinatorial Chemistry

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Nucleic Acid Selection and the Challenge of Combinatorial Chemistry

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