What question did this study set out to answer?

To establish a nondestructive technique for monitoring intracellular conditions, specifically pH, in erythrocytes using phosphorus-31 magnetic resonance spectroscopy.

October 1, 1973Journal of Biological ChemistryOpen Access

Determination of Intracellular pH by 31P Magnetic Resonance

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To establish a nondestructive technique for monitoring intracellular conditions, specifically pH, in erythrocytes using phosphorus-31 magnetic resonance spectroscopy.
Observed phosphorus-31 magnetic resonance signals from intracellular phosphate compounds in erythrocytes.
Measured chemical shifts of intracellular inorganic phosphate relative to serum phosphate and evaluated chemical shift positions and differences for 2,3-diphosphoglycerate in carbon monoxide-liganded erythrocytes.
The chemical shift of intracellular inorganic phosphate relative to serum phosphate reliably tracked intracellular pH changes.
Differences between the chemical shifts of 2,3-diphosphoglycerate provided a robust, nondestructive metric for measuring internal erythrocyte pH.

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Implication

Experimental study demonstrates nondestructive intracellular pH monitoring in erythrocytes using phosphorus-31 magnetic resonance, indicating a practical method to assess cell status.

Cite This Study

Moon et al. (1973) studied this question.

synapsesocial.com/papers/6a19a2fe3e4c9aaeb7f64e32 https://doi.org/10.1016/s0021-9258(19)43389-9

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Implication

Experimental study demonstrates nondestructive intracellular pH monitoring in erythrocytes using phosphorus-31 magnetic resonance, indicating a practical method to assess cell status.

Determination of Intracellular pH by 31P Magnetic Resonance

Key Points

Discussion

Implication

Cite This Study

Determination of Intracellular pH by 31P Magnetic Resonance

Key Points

Discussion

Implication

Cite This Study