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Abstract Two Gram-negative, moderately halophilic, and motile rod bacteria, strains G2-23 T and J2-29 T , showing catalase- and oxidase-positive activities were isolated from marine algae. Both strains optimally grew at 30°C, pH 7.0, and 2% (w/v) NaCl. Both strains contained ubiquinone-10 as the sole isoprenoid quinone. As major cellular fatty acids and polar lipids, strain G2-23 T contained summed feature 8 (C 18:1 ω 7 c and/or C 18:1 ω 6 c ), C 16:0 , and summed feature 3 (iso-C 15:0 2-OH and/or C 16:1 ω 7 c / ω 6 c ) and phosphatidylethanolamine (PE), phosphatidyl- N -monomethylethanolamine (PME), phosphatidylglycerol (PG), diphosphatidylglycerol, sulfoquinovosyl diacylglycerol (SQDG), and an unidentified phospholipid (PL), respectively, while strain J2-29 T contained summed feature 8, C 18:1 ω 7 c 11-methyl, and C 16:0 and PE, PME, PG, SQDG, and PL, respectively. The genomic DNA G + C contents of strains G2-23 T and J2-29 T were 59.5 and 62.2 mol%, respectively. Both strains shared 97.9% 16S rRNA gene sequence similarity, 79.8% average nucleotide identity (ANI), and 22.8% digital DNA-DNA hybridisation (DDH) value, indicating that they are different species. Phylogenetic and phylogenomic analyses by 16S rRNA gene and genome sequences, respectively, revealed that strains G2-23 T and J2-29 T formed different phylogenic lineages within the genus Hoeflea . ANI and digital DDH values between strains G2-23 T and J2-29 T and other Hoeflea type strains were less than 79.0 and 22.1% and 80.5 and 23.3%, respectively, representing that they are novel species of the genus Hoeflea . Based on the phenotypic, chemotaxonomic, and molecular properties, strains G2-23 T and J2-29 T represent two different novel species of the genus Hoeflea , for which the names Hoeflea algicola sp. nov. (G2-23 T = KACC 22714 T = JCM 35548 T ) and Hoeflea ulvae sp. nov. (J2-29 T = KACC 22715 T = JCM 35549 T ), respectively, are proposed.
Kristyanto et al. (Tue,) studied this question.