Lacibacter luteus sp. nov., isolated from a freshwater river

An isolate, designated TTM-7 T , recovered from a freshwater river in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain TTM-7 T is affiliated with species in the genus Lacibacter . Strain TTM-7 T was most closely related to Lacibacter cauensis NJ-8 T with a 98.2 % 16S rRNA gene sequence similarity, followed by Lacibacter daechungensis H32-4 T (97.4 %) and Lacibacter nakdongensis SS2-56 T (96.3 %). Cells were Gram-stain-negative, aerobic, motile by gliding, rod-shaped and formed orange colonies. Optimal growth occurred at 25 °C, pH 7 and 0 % NaCl. The major fatty acids of strain TTM-7 T were iso-C 15 : 0 , summed feature 3 (comprising C 16 : 1 ω 7 c/C 16 : 1 ω 6c and/or iso-C 15 : 0 2-OH) and iso-C 15 : 1 G. The predominant hydroxy fatty acid was iso-C 17 : 0 3-OH. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, two unidentified aminoglycolipids, five unidentified aminophospholipids and one unidentified lipid. The major isoprenoid quinone was MK-7. Genomic DNA G+C content of strain TTM-7 T was 40.6 mol%. Strain TTM-7 T showed 83.6 % average nucleotide identity and 16.0 % digital DNA–DNA hybridization identity with Lacibacter cauensis NJ-8 T . On the basis of phenotypic and genotypic properties and phylogenetic inference, strain TTM-7 T should be classified in a novel species of the genus Lacibacter , for which the name Lacibacter luteus sp. nov. is proposed. The type strain is TTM-7 T (=BCRC 81159 T =LMG 30926 T =KCTC 62870 T ).