What question did this study set out to answer?

To evaluate how spontaneous interleukin-10 expression by tumor cells suppresses GM-CSF-mediated cross-priming and antitumor immunity.

July 1, 1997The Journal of ImmunologyOpen Access

Interleukin-10 prevents dendritic cell accumulation and vaccination with granulocyte-macrophage colony-stimulating factor gene-modified tumor cells

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Authors

ZQZhihai QinWuhan Polytechnic University GNGabriele NoffzMemorial Sloan Kettering Cancer Center MMMariette MohauptMiltenyi Biotec (Germany)

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Implication

Experimental study reveals that tumor-derived interleukin-10 blocks dendritic cell recruitment and GM-CSF vaccine efficacy in mice, suggesting a mechanism of cancer immune evasion.

Key Points

To evaluate how spontaneous interleukin-10 expression by tumor cells suppresses GM-CSF-mediated cross-priming and antitumor immunity.
Engineered murine J558L tumor cells to express GM-CSF and evaluated their protective efficacy as whole-cell vaccines against parental tumor challenge.
Inhibited tumor-derived IL-10 expression using an IL-10 antisense retrovirus to determine its role in vaccine failure.
Conducted immunohistochemical analysis of vaccine sites to monitor the accumulation of MHC class II+ and DEC-205+ dendritic cells.
Immunization with GM-CSF-producing J558L cells failed to protect against challenge with the parental IL-10-producing tumor.
Inhibition of IL-10 expression with an antisense retrovirus restored the antitumor vaccine efficacy of GM-CSF-producing tumor cells.
Immunohistochemistry demonstrated that IL-10 completely blocked GM-CSF-induced dendritic cell accumulation (MHC class II+ and DEC-205+) at the vaccination site.

Cite This Study

Qin et al. (1997) studied this question.

synapsesocial.com/papers/6a19b8f91d4d911c80ea8a33 https://doi.org/10.4049/jimmunol.159.2.770

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Implication

Experimental study reveals that tumor-derived interleukin-10 blocks dendritic cell recruitment and GM-CSF vaccine efficacy in mice, suggesting a mechanism of cancer immune evasion.

Interleukin-10 prevents dendritic cell accumulation and vaccination with granulocyte-macrophage colony-stimulating factor gene-modified tumor cells

Discussion

Member takes

Implication

Key Points

Cite This Study

Interleukin-10 prevents dendritic cell accumulation and vaccination with granulocyte-macrophage colony-stimulating factor gene-modified tumor cells

Discussion

Member takes

Implication

Key Points

Cite This Study

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