What is the clinical evidence from this study?

Population: Vascular aging and endothelial dysfunction (n=110). Intervention: Aging (40 weeks vs 13 weeks) vs. Juvenile mice (13 weeks). Primary outcome: Vasorelaxation response to acetylcholine (ACh) and soluble guanylyl cyclase (sGC) activation (p=<0.05).

What does this research mean for the field?

Aging in mice compromises vasorelaxation and impairs vascular smooth muscle soluble guanylyl cyclase (sGC) function, accompanied by lower cGMP concentrations and reduced sGC mRNA expression, even before overt histopathological changes occur. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

October 22, 2021International Journal of Molecular SciencesOpen Access

Age Impairs Soluble Guanylyl Cyclase Function in Mouse Mesenteric Arteries

Q: What are the key findings of this study?

Aging impairs soluble guanylyl cyclase function and significantly reduces acetylcholine-induced vasorelaxation in mouse mesenteric arteries prior to the onset of overt histopathological alterations.

Q: What does this research mean for the field?

Aging in mice compromises vasorelaxation and impairs vascular smooth muscle soluble guanylyl cyclase (sGC) function, accompanied by lower cGMP concentrations and reduced sGC mRNA expression, even before overt histopathological changes occur. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

View Full Paper

Ask AI

Bookmark

Key result

Aging impairs mesenteric vasorelaxation by disrupting sGC function before overt structural vascular changes appear.

P<0.05
n=110

Why the study?

Endothelial dysfunction occurs with age without overt vessel damage, leading to the hypothesis that aging affects downstream signaling of the endothelial nitric oxide system in vascular smooth muscle.

Does aging impair soluble guanylyl cyclase function and vasorelaxation in mouse mesenteric arteries?

Population

Resistance mesenteric arteries isolated from 13-week and 40-week-old mice

Comparison

13-week (juvenile) vs 40-week-old (aged) mouse vessels

Design

Preclinical wire myography and histological study

Authors

CZCheng ZhongSouth China Agricultural University MXMinze XuHumboldt-Universität zu Berlin ŞBŞengül BoralCharité - Universitätsmedizin Berlin

Discussion

Member takes

Implication

Aging-related sGC impairment in mouse arteries is hypothesis-generating; leaves open human translation and therapeutic targeting before practice change.

Structured PICO

Does aging impair soluble guanylyl cyclase function and vasorelaxation in mouse mesenteric arteries?

Population

110 male C57BL/6 mice, comprising 65 juvenile (13 weeks) and 45 aged (40 weeks) animals, studied to assess age-related changes in mesenteric artery function.

Exposure

Ex vivo pharmacological testing of isolated mesenteric arteries (acetylcholine, L-NAME, sodium nitroprusside, runcaciguat, sildenafil) to assess aging effects

Comparator

Juvenile mice (13 weeks old)

Outcome

Vasorelaxation response (endothelium-dependent and independent) and soluble guanylyl cyclase (sGC) function/expressionsurrogate

Main Result

p-value: p=<0.05

Aging impairs vasorelaxation in mouse mesenteric arteries early in life due to blunted soluble guanylyl cyclase activity in vascular smooth muscle, even before overt histopathological changes occur.

Limitations

Protein expression data were not provided due to low specificity of available antibodies for sGC subtypes and PDEs.
The contribution of eNOS to vasorelaxation with respect to age requires further investigation.
Findings from this specific animal model may not fully account for variations in nutrition, motor activity, and environmental conditions seen in other aging models.

Cite This Study

Zhong et al. (2021) studied Vascular aging and endothelial dysfunction (n=110). Aging (40 weeks vs 13 weeks) vs. Juvenile mice (13 weeks) was evaluated on Vasorelaxation response to acetylcholine (ACh) and soluble guanylyl cyclase (sGC) activation (p=<0.05). Aging impairs soluble guanylyl cyclase function and significantly reduces acetylcholine-induced vasorelaxation in mouse mesenteric arteries prior to the onset of overt histopathological alterations.

synapsesocial.com/papers/6a19c11b407564563bf67f51 https://doi.org/10.3390/ijms222111412

View Full Paper

Ask AI

Bookmark

Implication

Aging-related sGC impairment in mouse arteries is hypothesis-generating; leaves open human translation and therapeutic targeting before practice change.

Age Impairs Soluble Guanylyl Cyclase Function in Mouse Mesenteric Arteries

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Age Impairs Soluble Guanylyl Cyclase Function in Mouse Mesenteric Arteries

Discussion

Member takes

Implication

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider