What question did this study set out to answer?

To examine the expression and cytokine regulation of CD40 on human endothelial cells and evaluate its functional capacity to trigger adhesion molecule expression.

May 9, 1995Proceedings of the National Academy of SciencesOpen Access

CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.

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To examine the expression and cytokine regulation of CD40 on human endothelial cells and evaluate its functional capacity to trigger adhesion molecule expression.
Quantified baseline and cytokine-stimulated CD40 levels on cultured human umbilical vein endothelial cells using monoclonal antibodies and fluorescence flow cytometry.
Treated endothelial cells with a trimeric recombinant CD40 ligand to measure adhesion molecule induction and assessed CD40 in normal human skin using immunocytochemistry.
Endothelial CD40 expression increased approximately 3-fold within 24 hours of exposure to TNF, IL-1, IFN-beta, or IFN-gamma, with interferons exhibiting synergistic effects when combined with TNF or IL-1.
CD40 ligand stimulation directly upregulated the leukocyte adhesion molecules E-selectin, VCAM-1, and ICAM-1 on endothelial cells, while CD40 was also identified in situ on normal dermal microvascular endothelium.

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Implication

In vitro analysis demonstrates cytokine-driven CD40 upregulation and adhesion molecule activation on human endothelial cells, suggesting an active signaling role during T-cell-mediated inflammation.

Cite This Study

Karmann et al. (1995) studied this question.

synapsesocial.com/papers/6a1a001c196cd56b09eb073b https://doi.org/10.1073/pnas.92.10.4342

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Implication

In vitro analysis demonstrates cytokine-driven CD40 upregulation and adhesion molecule activation on human endothelial cells, suggesting an active signaling role during T-cell-mediated inflammation.

CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.

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Discussion

Implication

Cite This Study

CD40 on human endothelial cells: inducibility by cytokines and functional regulation of adhesion molecule expression.

Key Points

Discussion

Implication

Cite This Study

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