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Identify the ligand for the B cell-associated antigen CD40 and evaluate its involvement in T cell-dependent activation of B lymphocytes.

Activated human T cells express a ligand for the human B cell‐associated antigen CD40 which participates in T cell‐dependent activation of B lymphocytes

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Identify the ligand for the B cell-associated antigen CD40 and evaluate its involvement in T cell-dependent activation of B lymphocytes.
Engineered a chimeric immunoglobulin molecule replacing the variable region with the extracellular domain of CD40 to detect ligand binding.
Stimulated human peripheral blood T cells with phorbol esters and ionomycin to measure surface kinetics and immunophenotype across CD4, CD8, and CD45 subsets.
Assayed the inhibitory effect of soluble CD40 on T cell-dependent B lymphocyte proliferation.
The chimeric CD40 protein specifically bound activated T cells and precipitated a 35-kDa surface ligand, appearing within 1 hour and reaching peak expression at 8 hours.
CD40 ligand expression occurred predominantly on CD4-positive T cells with minor expression on CD8-positive cells, displaying no correlation with CD45 phenotype.
Soluble CD40 functionally blocked T cell-dependent B cell proliferation.

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In vitro study demonstrates a 35-kDa CD40 ligand on activated human T cells, highlighting its essential role in driving B cell proliferation.

Lane et al. (1992) studied this question.

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In vitro study demonstrates a 35-kDa CD40 ligand on activated human T cells, highlighting its essential role in driving B cell proliferation.