What question did this study set out to answer?

To determine how recombinant murine CD40 ligand influences B cell costimulatory activity for CD4+ T cell proliferation and identify the specific surface molecules mediating this response.

January 1, 1994European Journal of Immunology

Induction of B cell costimulatory function by recombinant murine CD40 ligand

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To determine how recombinant murine CD40 ligand influences B cell costimulatory activity for CD4+ T cell proliferation and identify the specific surface molecules mediating this response.
Cultured murine B cells with recombinant membrane-bound CD40 ligand (CD40L), lipopolysaccharide (LPS), or control media.
Evaluated the capacity of activated B cells to stimulate CD4+ T cell proliferation in anti-CD3- or alloantigen-dependent assays.
Assessed surface expression and functional contribution of B7 and heat-stable antigen (HSA) using blocking agents CTLA4.Fc and 20C9 antibody.
Stimulation with CD40L or LPS induced B cells to strongly costimulate CD4+ T cell proliferation compared with control cultures.
LPS activation upregulated both B7 and HSA, with its costimulatory activity being completely blocked by combined CTLA4.Fc and 20C9 treatment.
CD40L activation upregulated B7 without enhancing HSA expression, and its costimulatory activity was only partially inhibited by combined CTLA4.Fc and 20C9.

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Implication

Experimental study demonstrates recombinant CD40 ligand induces B cell costimulatory activity in murine lymphocytes, suggesting a distinct B7-independent activation pathway.

Cite This Study

Kennedy et al. (1994) studied this question.

synapsesocial.com/papers/6a1ff7c435281a23f90db608 https://doi.org/10.1002/eji.1830240118

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Implication

Experimental study demonstrates recombinant CD40 ligand induces B cell costimulatory activity in murine lymphocytes, suggesting a distinct B7-independent activation pathway.

Induction of B cell costimulatory function by recombinant murine CD40 ligand

Key Points

Discussion

Implication

Cite This Study

Induction of B cell costimulatory function by recombinant murine CD40 ligand

Key Points

Discussion

Implication

Cite This Study

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