Biodata Mining of Differentially Expressed Genes between Acute Myocardial Infarction and Unstable Angina Based on Integrated Bioinformatics

Figures

• 

  Figure 1. Integrated bioinformatics-based technical route for differential gene research between acute myocardial infarction and unstable angina pectoris.

• 

  Figure 2. Heatmaps and volcano plots of differentially expressed genes between GSE29111 and GSE60993. (a) Heatmap of upregulated and downregulated genes of GSE29111. Each column represents a dataset, and each row represents a gene. Blue represents the downregulated gene, and red represents the upregulated gene. (b) Volcano plot of GSE29111. Blue represents the downregulated gene, red represents the upregulated gene, and gray represents the remaining differentially expressed genes. (c) Heatmap of upregulated and downregulated genes of GSE60993. (d) Volcano plot of GSE60993.

• 

  Figure 3. Protein-protein interaction (PPI) network and hub clustering modules of GSE29111. (a) The PPI network of DEGs. (b) Module 1 (MCODE score = 7). (c) Module 2 (MCODE score = 3). Blue represents the downregulated gene, and red represents the upregulated gene. The size of the node is proportional to the degree. (d) The connectivity map of 10 hub genes. From yellow to red, the darker the color, the higher the MCC score.

• 

  Figure 4. Protein-protein interaction (PPI) network and hub clustering modules of GSE60993. (a) The PPI network of DEGs. (b) Module 1 (MCODE score = 4). Blue represents the downregulated gene, and red represents the upregulated gene. The size of the node is proportional to the degree. (c) The connectivity map of 10 core genes. From yellow to red, the darker the color, the higher the MCC score.

• 

  Figure 5. GO and KEGG pathway enrichment analysis of DEGs in GSE29111. (a) Circular plot for GO enrichment analysis of DEGs. The inner circle represents the z-score, which is the downregulation of enriched genes under a certain GO entry. The outer circle represents GO item ID. The points in the outer ring represent the distribution of downregulated genes involved in this item. (b) Chord plot for GO enrichment analysis of DEGs. The right side represents GO entries, and different colors represent different GO entries. The left side represents genes enriched in GO entries. (c) KEGG pathway enrichment analysis of DEGs. The horizontal axis represents the rich factor, and the vertical axis represents the KEGG pathway.

• 

  Figure 6. GO and KEGG pathway enrichment analysis of DEGs in GSE60993. (a) GO enrichment analysis of DEGs. (b) KEGG pathway enrichment analysis of DEGs. The horizontal axis represents gene ratio, which is the ratio of the number of genes associated with the pathway to the total number of DEGs. The vertical axis represents the KEGG pathway.

• 

  Figure 7. Expression level analysis of key genes in GSE29111 and GSE60993. (a) Expression analysis of GSE29111 key genes in the AMI and UA groups. (b) Bitmap for correlation analysis of key genes in GSE29111. (c) Expression analysis of GSE60993 key genes in the AMI and UA groups. (d) Bitmap for correlation analysis of key genes in GSE60993. ns: there is no statistically significant difference between groups. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001.