MoS₂ exhibits stronger toxicity with increased exfoliation

MoS₂ belong to a class of inorganic 2D nanomaterials known as transition metal dichalcogenides (TMDs) which have recently attracted a renewed and growing interest due to their interesting electronic and catalytic properties when scaled down to single or few layer sheets. Although exfoliated MoS₂ nanosheets have been proposed for numerous energy-related and biosensing applications, little is known about the toxicological impacts of using MoS₂ nanosheets. Here, we report about the in vitro toxicity of MoS₂ nanosheets that have been chemically exfoliated with different lithium intercalating agents and compared their respective cytotoxic influence. Methyllithium (Me-Li), n-butyllithium (n-Bu-Li) and tert-butyllithium (t-Bu-Li) were used for the exfoliation of bulk MoS₂ and we found the t-Bu-Li and n-Bu-Li exfoliated MoS₂ nanosheets to be more cytotoxic than MoS₂ exfoliated by Me-Li. t-Bu-Li and n-Bu-Li provide more efficient exfoliation over Me-Li, and we establish that the extent of exfoliation that MoS₂ undergo is a factor influencing their toxicity. Specifically, the more exfoliated the MoS₂ nanosheets, the stronger its cytotoxic influence, which may be due to an increase in surface area and active edge sites. The potential toxicity of these MoS₂ nanosheets should be taken into account before their employment in real world applications and we have shown the effect the amount of exfoliation can have on the toxicity of MoS₂ nanosheets, representing the first step towards a better understanding of their toxicological properties.