Estrogen Inhibits Cardiomyocyte Hypertrophy in Vitro

Q: What does this research mean for the field?

17β-estradiol (E2) inhibits cardiomyocyte hypertrophy by restraining calcineurin and kinase signaling pathways, mediated through the induction of MCIP1 and stimulation of atrial natriuretic peptide production. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

Ali Pedram; Mahnaz Razandi; Mark Aitkenhead; Ellis R. Levin

doi:10.1074/jbc.m414409200

May 18, 2005Journal of Biological ChemistryOpen Access

Estrogen Inhibits Cardiomyocyte Hypertrophy in Vitro

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Why the study?

Does 17β-estradiol prevent angiotensin II- or endothelin-1-induced hypertrophy in cultured rat cardiomyocytes?

Key Points

The aim is to explore the mechanisms by which estrogen inhibits cardiomyocyte hypertrophy.
Cultured rat cardiomyocytes were treated with angiotensin II or endothelin-1, with and without estrogen.
Protein synthesis and calcineurin activity were measured using specific assays.
Gene expression related to calcineurin activity was analyzed via siRNA and RT-PCR.
Estrogen significantly reduced angiotensin II- or endothelin-1-induced protein synthesis and hypertrophy markers.
Calcineurin activity was inhibited by estrogen, with a corresponding upregulation of the MCIP1 gene.
Estrogen prevented the translocation of NF-AT to the nucleus, an essential step in the hypertrophic response.

Structured PICO

Does 17β-estradiol prevent angiotensin II- or endothelin-1-induced hypertrophy in cultured rat cardiomyocytes?

P

Population

Cultured rat neonatal cardiomyocytes isolated from 1-to-3-day-old rats or pregnant female rats

I

Intervention

17β-estradiol (E2) 10 nmol/liter

C

Comparator

Angiotensin II (100 nmol/liter) or endothelin-1 (10 nmol/liter) alone without E2

O

Outcome

Cardiomyocyte hypertrophy (measured by new protein synthesis, skeletal muscle actin expression, and increased surface area)surrogate

17β-estradiol inhibits cardiomyocyte hypertrophy in vitro by restraining calcineurin and kinase signaling pathways.

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Implication

E2 may inhibit AngII/ET-1-induced hypertrophy in rat cardiomyocytes; leaves open translation to human remodeling or therapy.

Abstract

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Cite This Study

Pedram et al. (2005) studied this question.

synapsesocial.com/papers/6a1a2ed8589f5a8c9bf6d218 https://doi.org/10.1074/jbc.m414409200

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Estrogen Inhibits Cardiomyocyte Hypertrophy in Vitro

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

Estrogen Inhibits Cardiomyocyte Hypertrophy in Vitro

Key Points

Structured PICO

Ask about this paper

Implication

Abstract

Discussion

Cite This Study

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