Integrated Network Pharmacology, Transcriptomics, and Experimental Validation Identify PI3K-AKT and STAT3 as Key Pathways for Pien Tze Huang Against Liver and Colorectal Cancers

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This study investigates the mechanisms of Pien Tze Huang (PZH) against liver and colorectal cancers, focusing on its active components and their targets.
Network pharmacology and transcriptomic analysis were conducted to identify active components and their targets for PZH, with validation using cell line assays.
Protein-Protein Interaction networks were constructed to screen core targets and pathways influenced by PZH in cancer.
Experimental validation was performed on Huh7, HCT116, and RKO cells, assessing cell viability, cycle progression, and gene expression.
PZH significantly reduced viability of Huh7 and HCT116 cells (1 mg/mL), indicating potent anti-proliferative effects.
RNA-sequencing revealed downregulation of proliferation-related genes including CCND1 and PD-L1 in RKO cells treated with PZH.
Key pathways PI3K-AKT and STAT3 were implicated in the inhibitory effects of PZH on cell cycle progression.

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Identifies PI3K-AKT and STAT3 pathways affected by Pien Tze Huang in liver and colorectal cancers, suggesting therapeutic potential.

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Hong et al. (2026) studied this question.

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