What question did this study set out to answer?

To determine the role of the pro-hormone secretogranin II in the biogenesis, maturation, and trafficking competence of dense core secretory granules in neuroendocrine cells.

January 9, 2010Journal of Biological ChemistryOpen Access

Pro-hormone Secretogranin II Regulates Dense Core Secretory Granule Biogenesis in Catecholaminergic Cells

View Full Paper

Ask AI

Bookmark

To determine the role of the pro-hormone secretogranin II in the biogenesis, maturation, and trafficking competence of dense core secretory granules in neuroendocrine cells.
Depleted secretogranin II expression in PC12 catecholaminergic cells to assess changes in dense core secretory granule morphology, count, and hormone trafficking.
Expressed secretogranin II fusion proteins in a secretory-deficient PC12 variant to evaluate rescue of the regulated secretory pathway, vesicular pH regulation, and exocytosis.
Secretogranin II depletion in PC12 cells resulted in a reduction in both the size and quantity of dense core secretory granules and impaired trafficking of regulated hormones.
Expression of secretogranin II fusion proteins rescued regulated exocytosis and restored dense core granules with acidic luminal pH maintained by V-type H(+)-ATPase, dependent on a trans-Golgi network pH gradient.

Discussion

No takes yet. Share an insight, caveat, or question.

Implication

Experimental study demonstrates that secretogranin II drives dense core secretory granule biogenesis in neuroendocrine cells, indicating pro-hormone aggregation orchestrates vesicle packaging.

Cite This Study

Courel et al. (2010) studied this question.

synapsesocial.com/papers/6a1aba4f49c6765e3885d942 https://doi.org/10.1074/jbc.m109.064196

View Full Paper

Ask AI

Bookmark

Implication

Experimental study demonstrates that secretogranin II drives dense core secretory granule biogenesis in neuroendocrine cells, indicating pro-hormone aggregation orchestrates vesicle packaging.

Pro-hormone Secretogranin II Regulates Dense Core Secretory Granule Biogenesis in Catecholaminergic Cells

Key Points

Discussion

Implication

Cite This Study

Pro-hormone Secretogranin II Regulates Dense Core Secretory Granule Biogenesis in Catecholaminergic Cells

Key Points

Discussion

Implication

Cite This Study

Also Consider

Also Consider