Key result
Destabilization of F-actin filaments by disulfide cross-linking or TMR labeling induces a tilted state in protomers similar to early polymerization, suggesting a time reversal of polymerization.
Population
F-actin filaments
Design
Preclinical
Authors
Loading...
Does not support clinical actin interventions; leaves open whether depolymerizing proteins reverse filament states in cardiovascular models.
F-actin filaments are annealed over time into a stable filament, and actin-depolymerizing proteins can effect a time reversal of polymerization.
Orlova et al. (2004) studied this question. Actin destabilization (disulfide cross-linking, TMR labeling) vs. Wild-type/control F-actin was evaluated on Proportion of protomers in a tilted state. Destabilization of F-actin filaments by disulfide cross-linking or TMR labeling induces a tilted state in protomers similar to early polymerization, suggesting a time reversal of polymerization.
Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context: