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October 1, 1992The Journal of Physiology210 citationsOpen Access

Stretch-Activated Anion Currents in Rabbit Cardiac Myocytes

Stretch‐activated anion currents of rabbit cardiac myocytes.

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Population

Rabbit cardiac myocytes (sino-atrial and atrial cells)

Comparison

Continuous application of positive pressure… vs Baseline or application of negative pressure…

Design

Preclinical

Authors

NHN HagiwaraHMHiroshi MasudaMSMorio Shoda

Discussion

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Overview

Animal data on rabbit Cl- currents warrant no clinical change; leaves open their role in human stretch-related arrhythmias.

Key Points

  • The study aims to investigate the properties and characteristics of stretch-activated anion currents in rabbit cardiac myocytes.
  • Utilized whole-cell patch clamp technique to measure stretch-activated currents in sino-atrial and atrial cells.
  • Applied positive pressure (5-15 cmH2O) to inflate cells and measured associated changes in membrane conductance.
  • Identified the sequence of anion permeability and the effects of various channel blockers on the currents.
  • The stretch-activated currents are Cl- selective, showing a reversal potential shifting with changes in intracellular Cl- concentration.
  • Anion permeability sequence determined as I-(1.7) > NO3-(1.5) > Br-(1.2) > Cl-(1.0) > F-(0.6), with SCN- exhibiting higher permeability than I-.
  • Stretch-activated Cl- current persists even when intracellular Ca2+ ions are chelated, indicating a unique modulation mechanism.

Structured PICO

P
Population
Rabbit cardiac myocytes (sino-atrial and atrial cells)
I
Intervention
Continuous application of positive pressure (5-15 cmH2O) through patch clamp electrode to induce cell inflation/stretch
C
Comparator
Baseline (before stretch) or application of negative pressure; various ion substitutions and pharmacological blockers
O
Outcome
Electrophysiological characteristics of stretch-activated currents (membrane conductance, reversal potential, ion selectivity, and response to blockers)surrogate

Identifies and characterizes a novel stretch-activated, Cl--selective current in rabbit cardiac myocytes that operates independently of intracellular calcium and protein kinases.

Cite This Study

Hagiwara et al. (1992) studied this question.

synapsesocial.com/papers/6a1aca838198c9a8aa461c99https://doi.org/10.1113/jphysiol.1992.sp019337
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Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

  1. 1Chloride‐sensitive nature of the adrenaline‐induced current in guinea‐pig cardiac myocytes.1990 · 120 citations
  2. 2Chloride current in mammalian cardiac myocytes. Novel mechanism for autonomic regulation of action potential duration and resting membrane potential.1990 · 147 citations
  3. 3Calcium-activated chloride current in rabbit ventricular myocytes.1991 · 275 citations
  4. 4Mechanotransducer ion channels in chick skeletal muscle: the effects of extracellular pH.1985 · 105 citations
  5. 5Isoquinolinesulfonamides, novel and potent inhibitors of cyclic nucleotide-dependent protein kinase and protein kinase C1984 · 2,705 citations