What question did this study set out to answer?

To examine whether the peptide bombesin stimulates DNA synthesis and cell proliferation in quiescent cultures of Swiss 3T3 cells.

May 1, 1983Proceedings of the National Academy of SciencesOpen Access

Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.

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To examine whether the peptide bombesin stimulates DNA synthesis and cell proliferation in quiescent cultures of Swiss 3T3 cells.
Treated confluent and quiescent Swiss 3T3 cells with bombesin in serum-free medium and in low-serum (3.5%) conditions.
Assayed dose- and time-dependent DNA synthesis alone and in combination with litorin, insulin, colchicine, PDGF, FDGF, vasopressin, phorbol esters, and cAMP-elevating agents.
Bombesin induced DNA synthesis in serum-free medium without other added growth factors, achieving half-maximal stimulation at 1 nM and driving proliferation in 3.5% serum.
Co-treatment with insulin, colchicine, PDGF, or FDGF enhanced the maximal response and reduced the half-maximal concentration of bombesin from 1 nM to 0.3 nM.
Litorin mimicked bombesin activity, whereas vasopressin, phorbol esters, and cAMP-increasing agents failed to potentiate bombesin-induced maximal DNA synthesis.

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Implication

In vitro study demonstrates potent mitogenic stimulation of DNA synthesis and cell division by bombesin in Swiss 3T3 cells, suggesting a model for ligand-controlled cell proliferation.

Cite This Study

Rozengurt et al. (1983) studied this question.

synapsesocial.com/papers/6a1acd4449c3147e1045f663 https://doi.org/10.1073/pnas.80.10.2936

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Implication

In vitro study demonstrates potent mitogenic stimulation of DNA synthesis and cell division by bombesin in Swiss 3T3 cells, suggesting a model for ligand-controlled cell proliferation.

Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.

Key Points

Discussion

Implication

Cite This Study

Bombesin stimulation of DNA synthesis and cell division in cultures of Swiss 3T3 cells.

Key Points

Discussion

Implication

Cite This Study

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