Frequency of DNA end joining in trans is not determined by the predamage spatial proximity of double-strand breaks in yeast

Significance DNA double-strand breaks (DSBs) are severe insults that lead to different types of genome rearrangements if repaired incorrectly. We explored the genesis of genomic rearrangements mediated by the nonhomologous end-joining pathway of DSB repair. Previous studies suggested that the efficiency of trans repair (i.e., rearrangements) is strongly influenced by the distance between two stretches of DNA incurring DSBs. We systematically investigated the impact of predamage spatial proximity on trans repair frequency in yeast and found no correlation between them, which has important implications for understanding chromosome movement and disease-causing rearrangements.