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When grown in the standard Dulbecco's medium the human liver derived Hep G2 hepatoma cell line shows only 10–20% of the cytochrome P‐450‐dependent mixed function oxidase (MFO) activity of freshly isolated human adult hepatocytes. However, the MFO activities and, to a lesser extent, the activities of UDP‐glucuronyltransferase and glutathione‐S‐transferase can be increased by altering the composition of the growth medium. Modified Earle's medium was more effective in this respect than Williams' E medium and increased the O ‐dealkylations of ethoxyresorufin, benzyloxyresorufin and pentoxyresorufin 50‐, 30‐ and 10‐fold, respectively.
Doostdar et al. (Mon,) studied this question.