What question did this study set out to answer?

To determine the molecular mechanism by which the Yersinia protein-tyrosine phosphatase YopH contributes to bacterial virulence and interacts with host cell substrates.

February 15, 1991Proceedings of the National Academy of SciencesOpen Access

Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant.

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Authors

JBJames B. BliskaDartmouth College KGKun‐Liang GuanUniversity of North Carolina at Chapel Hill JDJack E. DixonRoswell Park Comprehensive Cancer Center

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Implication

Laboratory study reveals YopH-dependent dephosphorylation of host proteins in macrophages and mice, indicating an essential enzymatic mechanism for Yersinia virulence.

Key Points

To determine the molecular mechanism by which the Yersinia protein-tyrosine phosphatase YopH contributes to bacterial virulence and interacts with host cell substrates.
Engineered a defined Cys-403 to Ala catalytic mutation into the plasmid-encoded yopH gene of Yersinia pseudotuberculosis using allelic recombination.
Assessed bacterial virulence in a murine infection model and quantified host protein dephosphorylation in infected macrophage monolayers using 32P-radiolabeling, immunoprecipitation, and SDS/PAGE.
Substitution of Cys-403 to Ala eliminated YopH phosphatase activity and significantly attenuated Y. pseudotuberculosis virulence in infected mice.
Wild-type bacteria dephosphorylated major 200-, 120-, and 60-kDa host macrophage proteins, with phosphate removal from the 120- and 60-kDa proteins strictly dependent on active YopH.
Host protein dephosphorylation occurred without bacterial phagocytosis, demonstrating that extracellular Yersinia delivers functional YopH to target host proteins.

Cite This Study

Bliska et al. (1991) studied this question.

synapsesocial.com/papers/6a1bc1711567d2fc4d5ee88b https://doi.org/10.1073/pnas.88.4.1187

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Implication

Laboratory study reveals YopH-dependent dephosphorylation of host proteins in macrophages and mice, indicating an essential enzymatic mechanism for Yersinia virulence.

Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant.

Discussion

Member takes

Implication

Key Points

Cite This Study

Tyrosine phosphate hydrolysis of host proteins by an essential Yersinia virulence determinant.

Discussion

Member takes

Implication

Key Points

Cite This Study

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