What question did this study set out to answer?

This research explores the role of nitric oxide in regulating angiogenesis in a chick embryo model.

April 1, 1993Open Access

Nitric oxide is involved in the regulation of angiogenesis

Key Points

This research explores the role of nitric oxide in regulating angiogenesis in a chick embryo model.
In vivo model using chick embryo chorioallantoic membrane (CAM)
Assessment of angiogenesis through collagenous protein biosynthesis and vascular density
Use of sodium nitroprusside and l-arginine to inhibit angiogenesis, and l-NMMA to promote it.
Sodium nitroprusside and l-arginine reduced vascular density and collagenous protein production.
L-NMMA increased both vascular density and collagenous protein biosynthesis, indicating promotion of angiogenesis.
Findings suggest that manipulation of nitric oxide synthesis could control angioproliferative diseases.

Abstract

The in vivo model of the chick embryo chorioallantoic membrane (CAM) was used to study the involvement of nitric oxide (NO) in angiogenesis. The nitrovasodilator sodium nitroprusside (NaNP) and the amino acid, L-arginine, inhibited angiogenesis, assessed as both collagenous protein biosynthesis and vascular density. NG-monomethyl-L-arginine (L-NMMA), an NO synthase inhibitor, increased both collagenous protein biosynthesis and vascular density, indicating that this agent promotes angiogenesis. These results suggest that NO may participate in the regulation of angiogenesis. Manipulation of NO synthesis therefore, may prove to be another approach for controlling angioproliferative diseases.

Nitric oxide is involved in the regulation of angiogenesis

Key Points

Abstract

Cite This Study