Ultra-Performance Liquid Chromatography (UPLC) has emerged as a powerful analytical technique in pharmaceutical sciences due to its superior resolution, speed, sensitivity, and reproducibility. The present study focuses on the significance of UPLC in pharmaceutical analysis, particularly in method development and validation for drug substances and dosage forms. The technique utilizes sub-2 µm particle columns and high operating pressures to achieve rapid and efficient separations while maintaining analytical accuracy and precision. Emphasis is placed on the role of quality control, regulatory compliance, and the need for validated analytical methods to ensure drug safety and efficacy. Various analytical techniques, including chromatographic and spectroscopic methods, were discussed, highlighting the dominant role of UPLC in modern laboratories. The study also elaborates on instrumentation components, working principles, advantages, and applications of UPLC in areas such as metabolite profiling, bioanalysis, and stability studies. Overall, UPLC represents a significant advancement over conventional chromatographic techniques, offering improved analytical performance and efficiency in pharmaceutical research and quality control.
Rajiv Kukkar2 Raghavendra S. V.*1 (Mon,) studied this question.
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