What question did this study set out to answer?

To test whether the voltage-clamp technique can provide a rapid, non-destructive electronic measurement of intracellular sodium concentration and net ionic flux in squid axons.

September 1, 1961The Journal of General PhysiologyOpen Access

Electronic Measurement of the Intracellular Concentration and Net Flux of Sodium in the Squid Axon

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Authors

JMJohn W. MooreUniversity of Arkansas at Little Rock WAWilliam J. AdelmanUniversity of Vermont

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Implication

Experimental study demonstrates non-destructive tracking of intracellular sodium concentration and flux in squid axons, indicating the viability of real-time electronic monitoring.

Key Points

To test whether the voltage-clamp technique can provide a rapid, non-destructive electronic measurement of intracellular sodium concentration and net ionic flux in squid axons.
Controlled squid axon membrane potential electronically using the voltage-clamp technique to determine the reversal potential of early ionic currents as a marker of internal sodium.
Tracked internal sodium changes over time in fresh resting axons, during rapid electrical stimulation of unclamped axons, and during voltage pulses driven above the sodium equilibrium potential.
Intracellular sodium concentration increased from 38 mM in fresh axons to 50 mM over roughly one hour, corresponding to an estimated resting net flux of 40 pmoles/cm² sec.
Rapid electrical stimulation produced a marked increase in the rate of sodium accumulation, whereas rapid voltage pulses positive to the sodium potential drove sodium outward to decrease internal concentration.

Cite This Study

Moore et al. (1961) studied this question.

synapsesocial.com/papers/6a1bd5771567d2fc4d5f1b1b https://doi.org/10.1085/jgp.45.1.77

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Implication

Experimental study demonstrates non-destructive tracking of intracellular sodium concentration and flux in squid axons, indicating the viability of real-time electronic monitoring.

Electronic Measurement of the Intracellular Concentration and Net Flux of Sodium in the Squid Axon

Discussion

Member takes

Implication

Key Points

Cite This Study

Electronic Measurement of the Intracellular Concentration and Net Flux of Sodium in the Squid Axon

Discussion

Member takes

Implication

Key Points

Cite This Study

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