What question did this study set out to answer?

To determine the role of the adaptor protein NHERF-1 in mediating dopamine inhibition of renal sodium-phosphate co-transport.

March 4, 2010Journal of Biological ChemistryOpen Access

Sodium-Hydrogen Exchanger Regulatory Factor 1 (NHERF-1) Transduces Signals That Mediate Dopamine Inhibition of Sodium-Phosphate Co-transport in Mouse Kidney

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Authors

EWEdward J. WeinmanUniversity of Maryland, Baltimore RBRajatsubhra BiswasUniversity of Maryland, Baltimore DSDeborah SteplockWest Virginia University

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Implication

Experimental study demonstrates that NHERF-1 mediates dopamine-induced inhibition of phosphate transport in mouse kidney cells, indicating an essential adaptor role.

Key Points

To determine the role of the adaptor protein NHERF-1 in mediating dopamine inhibition of renal sodium-phosphate co-transport.
Assayed phosphate transport in isolated renal brush border membrane vesicles and cultured renal proximal tubule cells from wild-type and NHERF-1 null mice.
Conducted co-immunoprecipitation, cAMP accumulation, and protein kinase C (PKC) assays alongside adenoviral rescue of NHERF-1 expression.
Measured NHERF-1 phosphorylation at serine 77 using 32P-labeling in mouse proximal tubule and opossum kidney cells.
Dopamine inhibited phosphate transport and stimulated cAMP accumulation and PKC activity in wild-type mice, but these responses were completely absent in NHERF-1 null mice.
Adenoviral reintroduction of NHERF-1 into deficient proximal tubule cells successfully restored dopamine-stimulated cAMP accumulation and PKC activation.
Dopamine treatment induced phosphorylation of NHERF-1 at serine 77 in its PDZ I domain, a modification known to weaken binding to the sodium-phosphate co-transporter Npt2a.

Cite This Study

Weinman et al. (2010) studied this question.

synapsesocial.com/papers/6a1bdf6826cb5670aa9d07e2 https://doi.org/10.1074/jbc.m109.094359

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Implication

Experimental study demonstrates that NHERF-1 mediates dopamine-induced inhibition of phosphate transport in mouse kidney cells, indicating an essential adaptor role.

Sodium-Hydrogen Exchanger Regulatory Factor 1 (NHERF-1) Transduces Signals That Mediate Dopamine Inhibition of Sodium-Phosphate Co-transport in Mouse Kidney

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Key Points

Cite This Study

Sodium-Hydrogen Exchanger Regulatory Factor 1 (NHERF-1) Transduces Signals That Mediate Dopamine Inhibition of Sodium-Phosphate Co-transport in Mouse Kidney

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Implication

Key Points

Cite This Study

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