What question did this study set out to answer?

To determine how varying relative fluxes of superoxide and nitric oxide modulate the chemical pathways governing thiol nitrosation versus oxidation using glutathione as a model.

April 1, 1997Journal of Biological ChemistryOpen Access

Superoxide Modulates the Oxidation and Nitrosation of Thiols by Nitric Oxide-derived Reactive Intermediates

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To determine how varying relative fluxes of superoxide and nitric oxide modulate the chemical pathways governing thiol nitrosation versus oxidation using glutathione as a model.
Exposed aqueous solutions of reduced glutathione to the nitric oxide donor Et2NN(O)NONa.
Introduced varying fluxes of superoxide via xanthine oxidase and evaluated mechanistic modulation using superoxide dismutase and catalase.
Addition of xanthine oxidase caused a concentration-dependent decline in S-nitrosoglutathione formation, reaching a 95% reduction when nitric oxide and superoxide fluxes were nearly equivalent.
Superoxide dismutase restored nitrosation while catalase had no effect, and oxidized glutathione formation reached peak levels when the fluxes of nitric oxide and superoxide were approximately 1:1.

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Implication

In vitro study shows that superoxide flux alters nitric oxide-mediated thiol modification from nitrosation to oxidation, highlighting a flux-dependent chemical regulatory switch.

Cite This Study

Wink et al. (1997) studied this question.

synapsesocial.com/papers/6a1be4579dc17f23939cc5f0 https://doi.org/10.1074/jbc.272.17.11147

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Implication

In vitro study shows that superoxide flux alters nitric oxide-mediated thiol modification from nitrosation to oxidation, highlighting a flux-dependent chemical regulatory switch.

Superoxide Modulates the Oxidation and Nitrosation of Thiols by Nitric Oxide-derived Reactive Intermediates

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Discussion

Implication

Cite This Study

Superoxide Modulates the Oxidation and Nitrosation of Thiols by Nitric Oxide-derived Reactive Intermediates

Key Points

Discussion

Implication

Cite This Study

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