What does this research mean for the field?

Phosphorylation of the M-domain reduces the functional potency of cardiac myosin-binding protein C (cMyBP-C) by compacting its structure, but this effect is antagonized and reversed by high calcium levels during peak contraction. Novelty: ClaimNovelty.NOVEL_FINDING. Consensus alignment: ConsensusAlignment.NEUTRAL.

February 23, 2016Proceedings of the National Academy of SciencesOpen Access

Phosphorylation and calcium antagonistically tune myosin-binding protein C’s structure and function

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Population

Cardiac myosin-binding protein C (cMyBP-C) N-terminal domains (M-domain) and mutant proteins

Comparison

Phosphorylation of the M-domain and varying… vs Unphosphorylated state and low calcium levels

Design

Preclinical

Authors

MPMichael J. PrevisUniversity of Vermont JMJi Young MunKorea Brain Research Institute AMArthur J. MichalekClarkson University

Discussion

Member takes

Implication

Animal data show calcium overrides phosphorylation effects on cMyBP-C; extends mechanistic insights but leaves human translation open.

Structured PICO

Population

Cardiac myosin-binding protein C (cMyBP-C) N-terminal domains (M-domain) and mutant proteins

Intervention

Phosphorylation of the M-domain and varying calcium levels

Comparator

Unphosphorylated state and low calcium levels

Outcome

M-domain extensibility, N-terminal domain conformation, and maximal sliding velocity in motility assayssurrogate

M-domain phosphorylation tunes cMyBP-C's calcium-sensitization primarily at low calcium levels between contractions, while peak calcium levels allow it to remain a potent contractile modulator regardless of phosphorylation state.

Cite This Study

Previs et al. (2016) studied this question.

synapsesocial.com/papers/6a1be651ea84844e355f2615 https://doi.org/10.1073/pnas.1522236113

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Phosphorylation and calcium antagonistically tune myosin-binding protein C’s structure and function

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Phosphorylation and calcium antagonistically tune myosin-binding protein C’s structure and function

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider