What does this research mean for the field?

The secondary structure of substrate RNA is a major determinant of hammerhead ribozyme catalytic efficiency, primarily affecting the Michaelis constant (Km) due to the formation of nonreactive substrate aggregates, while the catalytic rate constant (kcat) remains largely unchanged. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

March 1, 1990Proceedings of the National Academy of SciencesOpen Access

Substrate sequence effects on "hammerhead" RNA catalytic efficiency.

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Population

"hammerhead" RNA self-cleaving domain assembled from two RNA molecules

Design

Preclinical

Authors

MFMartha J. FedorScripps Research Institute OUOlke C. UhlenbeckNorthwestern University

Discussion

Member takes

Implication

Substrate structure may dictate hammerhead efficiency in vitro; leaves open relevance to therapeutic ribozyme applications.

Structured PICO

Population

"hammerhead" RNA self-cleaving domain assembled from two RNA molecules (large ribozyme RNA and small substrate RNA)

Intervention

Four hammerheads with identical catalytic core sequences but differing base composition of helices involved in substrate binding

Outcome

Cleavage rates (kcat and Km values)surrogate

The secondary structure of substrate RNA can be a major determinant of hammerhead catalytic efficiency, as substrates with high Km values form nonreactive aggregates.

Cite This Study

Fedor et al. (1990) studied this question.

synapsesocial.com/papers/6a1bef25ea84844e355f314e https://doi.org/10.1073/pnas.87.5.1668

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March 1, 1990Proceedings of the National Academy of SciencesOpen Access

Substrate sequence effects on "hammerhead" RNA catalytic efficiency.

View Full Paper

Ask AI

Bookmark

Population

"hammerhead" RNA self-cleaving domain assembled from two RNA molecules

Design

Preclinical

Authors

MFMartha J. FedorScripps Research Institute OUOlke C. UhlenbeckNorthwestern University

Discussion

Member takes

Implication

Substrate structure may dictate hammerhead efficiency in vitro; leaves open relevance to therapeutic ribozyme applications.

Structured PICO

Population

"hammerhead" RNA self-cleaving domain assembled from two RNA molecules (large ribozyme RNA and small substrate RNA)

Intervention

Four hammerheads with identical catalytic core sequences but differing base composition of helices involved in substrate binding

Outcome

Cleavage rates (kcat and Km values)surrogate

The secondary structure of substrate RNA can be a major determinant of hammerhead catalytic efficiency, as substrates with high Km values form nonreactive aggregates.

Cite This Study

Fedor et al. (1990) studied this question.

synapsesocial.com/papers/6a1bef25ea84844e355f314e https://doi.org/10.1073/pnas.87.5.1668

Also Consider

Synapse has enriched 4 closely related papers on similar clinical questions. Consider them for comparative context:

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