Identification of transcription factors MYC and C/EBPβ mediated regulatory networks in heart failure based on gene expression omnibus datasets

Figures

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  Fig. 1. Search strategies used for accessing the gene datasets was described in the flowchart

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  Fig. 2. Identification of the transcriptomic features of heart failure. a Un-supervised clustering heatmaps represented the differentially expressed genes (P < 0.01) in failing heart tissues compared to normal heart tissues in GSE5406, GSE16499 and GSE68316 datasets. Each column represented one sample. Genes up-regulated (red), down-regulated (blue) and moderately regulated (black) were delineated. Number of sample in each dataset was also showed. b Venn diagrams depicted the overlapped differentially expressed genes in GSE5406, GSE16499 and GSE68316 datasets. Red cycle represented the up regulated genes in failing heart tissues and green cycle represented the down regulated genes in failing heart tissues

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  Fig. 3. Metabolism and insulin signaling pathway are suppressed in failing heart patients. a Functional pathways enrichment analysis of the common differentially expressed 90 genes in failing heart tissues. b Differentially expressed genes from cysteine, methionine, pyrimidine and purine metabolism signaling pathway were represented using heatmaps. c Enrichment plots showed the enriched insulin signaling pathway in GSE5406, GSE16499 and GSE68316 datasets. Enrichment of NES and P values were shown. d Box plots showed the expression levels of MAP2K1 in GSE5406, GSE16499 and GSE68316 datasets. P values showed the difference of MAP2K1 expression levels between failing heart tissues and normal heart tissues determined by Student’s t test

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  Fig. 4. Transcription factors MYC and C/EBP are negatively associated with in failing heart expression profiling. a Functional transcription factors enrichment analysis of the common differentially expressed 90 genes in failing heart tissues. b Enrichment plots of transcription factor MYC in GSE5406, GSE16499 and GSE68316 datasets. Enrichment of NES and P values were shown. c Enrichment plots of transcription factor C/EBP in GSE5406, GSE16499 and GSE68316 datasets

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  Fig. 5. Transcription factors MYC and C/EBPβ are down regulated in failing heart tissues. a Box plots showed the transcription factor MYC expression levesl. P values showed the difference of genes expression levels between failing heart and normal heart tissues determined by Student’s t test. b Box plots showed the transcription factor C/EBPβ expression levels. c Pearson correlation between MYC and C/EBPβ expression levels in GSE5406 and GSE16499 datasets. Adjusted R-square and P value were shown

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  Fig. 6. MYC and C/EBPβ target genes are down regulated in failing heart tissues. a Heatmap demonstrated the expression profile of MYC target genes in failing heart and normal heart tissues in GSE16499 dataset. Genes up-regulated (red), down-regulated (blue) and moderately regulated (black) were delineated. b Heatmap demonstrated the expression profile of C/EBPβ target genes in failing heart and normal heart tissues in GSE16499 dataset. c Venn diagram depicted the four overlapped MYC and C/EBPβ target genes. d Box plots showed the expression levels of SYNCRIP

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  Fig. 7. The MYC and C/EBPβ mediated transcriptional networks. a MYC mediated regulatory network was created by cytoscape using MYC target genes. The red ones implied that those genes were previously reported involving the development of heart failure. b C/EBPβ mediated regulatory network was created by cytoscape using C/EBPβ target genes