What question did this study set out to answer?

To determine the relationship between cell body and axonemal alpha-tubulin forms and characterize the site and timing of alpha-tubulin posttranslational modification during flagellar assembly.

July 1, 1983The Journal of Cell BiologyOpen Access

Chlamydomonas alpha-tubulin is posttranslationally modified in the flagella during flagellar assembly.

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Authors

SLSteven W. L’HernaultEmory University JRJoel L. RosenbaumThe Ohio State University

Discussion

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Implication

Biochemical study demonstrates posttranslational modification of alpha-tubulin during flagellar assembly in Chlamydomonas, indicating localized protein maturation within the flagellum.

Key Points

To determine the relationship between cell body and axonemal alpha-tubulin forms and characterize the site and timing of alpha-tubulin posttranslational modification during flagellar assembly.
Examined isoelectric variants of alpha-tubulin across the cell body, flagellar matrix, and axoneme fractions in Chlamydomonas reinhardtii.
Induced flagellar assembly in the absence of de novo protein synthesis and performed in vivo metabolic radiolabeling using tritiated acetate.
Precursor alpha-tubulin from the cell body enters the flagellar matrix fraction and undergoes posttranslational conversion to the axonemal form prior to or during incorporation into microtubules.
In vivo labeling with tritiated acetate during protein synthesis-inhibited flagellar regeneration selectively labeled alpha-tubulin without detectable incorporation into other flagellar polypeptides.

Cite This Study

L’Hernault et al. (1983) studied this question.

synapsesocial.com/papers/6a1bf224ea84844e355f363e https://doi.org/10.1083/jcb.97.1.258

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July 1, 1983The Journal of Cell BiologyOpen Access

Chlamydomonas alpha-tubulin is posttranslationally modified in the flagella during flagellar assembly.

View Full Paper

Ask AI

Bookmark

Authors

SLSteven W. L’HernaultEmory University JRJoel L. RosenbaumThe Ohio State University

Discussion

Member takes

Implication

Biochemical study demonstrates posttranslational modification of alpha-tubulin during flagellar assembly in Chlamydomonas, indicating localized protein maturation within the flagellum.

Key Points

To determine the relationship between cell body and axonemal alpha-tubulin forms and characterize the site and timing of alpha-tubulin posttranslational modification during flagellar assembly.
Examined isoelectric variants of alpha-tubulin across the cell body, flagellar matrix, and axoneme fractions in Chlamydomonas reinhardtii.
Induced flagellar assembly in the absence of de novo protein synthesis and performed in vivo metabolic radiolabeling using tritiated acetate.
Precursor alpha-tubulin from the cell body enters the flagellar matrix fraction and undergoes posttranslational conversion to the axonemal form prior to or during incorporation into microtubules.
In vivo labeling with tritiated acetate during protein synthesis-inhibited flagellar regeneration selectively labeled alpha-tubulin without detectable incorporation into other flagellar polypeptides.

Cite This Study

L’Hernault et al. (1983) studied this question.

synapsesocial.com/papers/6a1bf224ea84844e355f363e https://doi.org/10.1083/jcb.97.1.258

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