What question did this study set out to answer?

To evaluate the sensitivity, specificity, and reproducibility of an immunodot enzymatic assay using purified glycoprotein G (gG-2) for detecting herpes simplex virus type 2 antibodies.

October 1, 1985Journal of Clinical MicrobiologyOpen Access

Detection of herpes simplex virus type 2-specific antibody with glycoprotein G

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Authors

FLFrancis K. LeeBipar RCRobert M. ColemanUniversity of Waterloo LPLenore PereiraUniversity of California, San Francisco

Discussion

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Implication

Diagnostic evaluation demonstrates high sensitivity and specificity of a glycoprotein G immunodot assay in human sera, highlighting its reliability for herpes simplex virus type 2 serology.

Key Points

To evaluate the sensitivity, specificity, and reproducibility of an immunodot enzymatic assay using purified glycoprotein G (gG-2) for detecting herpes simplex virus type 2 antibodies.
Purified gG-2 using a monoclonal antibody immunoaffinity column and applied it onto nitrocellulose paper for an enzymatic immunodot assay.
Tested serum samples from 134 individuals with culture-confirmed recurrent genital HSV-2 and 631 control individuals (245 seronegative adults, 344 children, 29 nuns, and 13 individuals with primary HSV-1).
Assessed assay reproducibility by retesting 391 serum samples and compared diagnostic specificity against standard microneutralization tests.
HSV-2 antibodies were detected in 132 of 134 (98.5%) convalescent sera from recurrent HSV-2 cases, including 17% of sera obtained within 10 days and 95% obtained more than 10 days after primary infection onset.
Assay specificity was 100%, with 0 of 631 control sera reacting to the gG-2 antigen, demonstrating higher specificity than microneutralization.
Test-retest reproducibility reached 95% across 391 re-examined serum samples.

Cite This Study

Lee et al. (1985) studied this question.

synapsesocial.com/papers/6a1bf87bb33628da419d100b https://doi.org/10.1128/jcm.22.4.641-644.1985

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Implication

Diagnostic evaluation demonstrates high sensitivity and specificity of a glycoprotein G immunodot assay in human sera, highlighting its reliability for herpes simplex virus type 2 serology.

Detection of herpes simplex virus type 2-specific antibody with glycoprotein G

Discussion

Member takes

Implication

Key Points

Cite This Study

Detection of herpes simplex virus type 2-specific antibody with glycoprotein G

Discussion

Member takes

Implication

Key Points

Cite This Study

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