Differential injection with radiopaque materials followed by projection x-ray microscopy has demonstrated the microangiographic pattern of epicerebral, transcerebral, and cortical blood vessels in the human brain. The authors report that alkaline phosphatase activity provides a means of showing the capillary bed more completely and differentiating cortical arteries from cortical veins. The vertical and horizontal organization of the cortical vessels is related to the cortical columns and lamination. Pairing and spiraling of arterioles of the penetrating cortical vessels are described. Lead staining for neuronal acid phosphatase activity in combination with alkaline phosphatase stain for blood vessels makes it possible to examine the neurons and microvascular pattern by both optical and projection x-ray microscopy.
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Saunders et al. (1971) studied this question.