Three-dimensional Cardiomyocytes Structure Revealed By Diffusion Tensor Imaging and Its Validation Using a Tissue-Clearing Technique
Key result
Diffusion tensor imaging accurately mapped three-dimensional cardiomyocytes orientation, demonstrating significant correlation (R2 0.803) with intact 3D histology acquired by a tissue-clearing technique in a mouse model.
Population
15 adult male C57/B6 mice (n=7 normal controls, n=8 ischemic heart failure model induced by LAD ligation)
Comparison
Diffusion tensor imaging and 3D histology using… vs Normal vs ischemic hearts; DTI compared to 3D…
Design
Preclinical
Follow-up
30 days
Authors
Discussion
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Member takes
Implication
Validates DTI for cardiomyocyte mapping in mouse hearts; extends preclinical imaging research but requires human validation.
Structured PICO
P
Population
15 adult male C57/B6 mice (n=7 normal controls, n=8 ischemic heart failure model induced by LAD ligation)
I
Intervention
Diffusion tensor imaging (DTI) and 3D histology using a tissue-clearing technique (CLARITY)
C
Comparator
Normal vs ischemic hearts; DTI compared to 3D optical imaging and 2D histology
O
Outcome
Cardiomyocyte orientation (Helix angle [HA] and global HA transmurality [HAT])surrogate
Main Result
Effect estimate: R2 0.803 (95% CI -0.33 to 0.24)
p-value: p=<0.001
DTI accurately maps 3D cardiomyocyte orientation and detects microstructural disruption in ischemic heart failure, validated by intact 3D tissue-clearing histology.
Limitations
- Agreement between conventional 2D histology and 3D histology using mirror section was good but not high due to tissue swelling and slice gaps.
- Sheetlet analysis could not be verified because the second and third eigenvectors of the structure tensor of the cleared tissue were difficult to distinguish.
- The study was conducted ex vivo, so serial imaging to detect temporal changes was not performed.
- Agreement between 2D and 3D histology was good but not high due to mirror sectioning gaps and tissue swelling
- Sheetlet analysis could not be verified
- Ex vivo study without serial imaging
Cite This Study
Lee et al. (2018) studied Ischemic heart failure (mouse model) (n=15). Diffusion Tensor Imaging (DTI) vs. 3D histology using tissue-clearing technique (CLARITY) was evaluated on Correlation of global Helix Angle Transmurality (HAT) between DTI and 3D histology (R2 0.803, 95% CI -0.33 to 0.24, p=<0.001). Diffusion tensor imaging accurately mapped three-dimensional cardiomyocytes orientation, demonstrating significant correlation (R2 0.803) with intact 3D histology acquired by a tissue-clearing technique in a mouse model.