What question did this study set out to answer?

Develop an automated, web-based computational tool to streamline the design, restriction site selection, and assembly preparation of TALEN constructs for genome editing.

January 16, 2013BMC BioinformaticsOpen Access

Mojo Hand, a TALEN design tool for genome editing applications

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Authors

KNKevin L. NeffMayo Clinic DADavid P. ArgueMayo Clinic in Arizona CAC. H. AlvinChinese University of Hong Kong

Discussion

Member takes

Implication

Software development study demonstrates automated design of custom TALEN constructs, facilitating more rapid and reliable genome editing workflows.

Key Points

Develop an automated, web-based computational tool to streamline the design, restriction site selection, and assembly preparation of TALEN constructs for genome editing.
Designed and deployed a web-based algorithm (Mojo Hand) that automates the retrieval of genomic sequence data from the National Center for Biotechnology Information.
Programmed candidate site searches to detect paired TAL monomer binding sites and identify intervening restriction enzyme recognition sites matched to commercial vendor catalogs.
Engineered automated output data formatting to guide downstream molecular cloning via the Golden Gate assembly method.
Mojo Hand systematically processes target DNA sequences to output validated TALEN binding pairs and spacer restriction enzyme cut sites.
The tool successfully integrates site predictions with reagent-tracking spreadsheets to simplify physical construct assembly and accelerate genome editing applications.

Cite This Study

Neff et al. (2013) studied this question.

synapsesocial.com/papers/6a1bfedd666b677c61a917da https://doi.org/10.1186/1471-2105-14-1

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January 16, 2013BMC BioinformaticsOpen Access

Mojo Hand, a TALEN design tool for genome editing applications

View Full Paper

Ask AI

Bookmark

Authors

KNKevin L. NeffMayo Clinic DADavid P. ArgueMayo Clinic in Arizona CAC. H. AlvinChinese University of Hong Kong

Discussion

Member takes

Implication

Software development study demonstrates automated design of custom TALEN constructs, facilitating more rapid and reliable genome editing workflows.

Key Points

Develop an automated, web-based computational tool to streamline the design, restriction site selection, and assembly preparation of TALEN constructs for genome editing.
Designed and deployed a web-based algorithm (Mojo Hand) that automates the retrieval of genomic sequence data from the National Center for Biotechnology Information.
Programmed candidate site searches to detect paired TAL monomer binding sites and identify intervening restriction enzyme recognition sites matched to commercial vendor catalogs.
Engineered automated output data formatting to guide downstream molecular cloning via the Golden Gate assembly method.
Mojo Hand systematically processes target DNA sequences to output validated TALEN binding pairs and spacer restriction enzyme cut sites.
The tool successfully integrates site predictions with reagent-tracking spreadsheets to simplify physical construct assembly and accelerate genome editing applications.

Cite This Study

Neff et al. (2013) studied this question.

synapsesocial.com/papers/6a1bfedd666b677c61a917da https://doi.org/10.1186/1471-2105-14-1

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