What question did this study set out to answer?

This work aims to identify and characterize a gene in Schizosaccharomyces pombe that encodes a protein related to protein-tyrosine-phosphatases.

A fission-yeast gene encoding a protein with features of protein-tyrosine-phosphatases.

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Gene pyp1+ encodes a protein similar to mammalian protein-tyrosine-phosphatases, suggesting evolutionary conservation.

This work aims to identify and characterize a gene in Schizosaccharomyces pombe that encodes a protein related to protein-tyrosine-phosphatases.
Utilized degenerate oligonucleotide probes to amplify DNA fragments from a cDNA library using PCR.
Cloned and sequenced the PCR product to identify the pyp1+ gene.
Assessed the effects of gene disruption and overexpression on protein detection.
The pyp1+ gene was identified and predicted to encode a 550-amino acid protein with conserved sequences similar to mammalian PTPases.
Disruption of the pyp1+ gene yielded viable S. pombe cells.
Overexpression of pyp1+ allowed the detection of a protein with a molecular weight of approximately 63,000.

Ottilie et al. (1991) studied this question.

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