July 1, 1989Journal of Biological ChemistryOpen Access

Site-directed Mutation of the Trigger Calcium-binding Sites in Cardiac Troponin C

View Full Paper

Ask AI

Bookmark

Population

Chicken cardiac troponin C cDNA expressed in a bacterial plasmid and skinned slow skeletal muscle fibers

Comparison

Site-directed mutagenesis activating… vs Wild type cardiac troponin C

Design

Preclinical

Authors

JPJohn A. PutkeyThe University of Texas Health Science Center at Houston HSH. Lee SweeneyHeart Failure & Transplant SCSean CampbellAlbert Einstein College of Medicine

Discussion

Member takes

Implication

No immediate clinical implications from this animal study; it leaves open troponin C site targeting for human contractility research.

Structured PICO

Population

Chicken cardiac troponin C cDNA expressed in a bacterial plasmid and skinned slow skeletal muscle fibers

Intervention

Site-directed mutagenesis activating Ca2+-binding site I (deletion of Val-28 and conversion of amino acids 29-32) and inactivating Ca2+-binding site II (mutation of Asp-65, Asp-67, and Gly-70)

Comparator

Wild type cardiac troponin C

Outcome

Ca2+-binding characteristics, Ca2+-dependent conformational changes (measured by fluorescence), and characteristics of contraction in skinned muscle fiberssurrogate

This study provides direct evidence that Ca2+-binding site II in cardiac troponin C is essential for triggering muscle contraction, while site I functions to modify contraction characteristics.

Cite This Study

Putkey et al. (1989) studied this question.

synapsesocial.com/papers/6a1c0bf94ebd09f3dfa96020 https://doi.org/10.1016/s0021-9258(18)63868-2

View Full Paper

Ask AI

Bookmark

July 1, 1989Journal of Biological ChemistryOpen Access

Site-directed Mutation of the Trigger Calcium-binding Sites in Cardiac Troponin C

View Full Paper

Ask AI

Bookmark

Population

Chicken cardiac troponin C cDNA expressed in a bacterial plasmid and skinned slow skeletal muscle fibers

Comparison

Site-directed mutagenesis activating… vs Wild type cardiac troponin C

Design

Preclinical

Authors

JPJohn A. PutkeyThe University of Texas Health Science Center at Houston HSH. Lee SweeneyHeart Failure & Transplant SCSean CampbellAlbert Einstein College of Medicine

Discussion

Member takes

Implication

No immediate clinical implications from this animal study; it leaves open troponin C site targeting for human contractility research.

Structured PICO

Population

Chicken cardiac troponin C cDNA expressed in a bacterial plasmid and skinned slow skeletal muscle fibers

Intervention

Site-directed mutagenesis activating Ca2+-binding site I (deletion of Val-28 and conversion of amino acids 29-32) and inactivating Ca2+-binding site II (mutation of Asp-65, Asp-67, and Gly-70)

Comparator

Wild type cardiac troponin C

Outcome

Ca2+-binding characteristics, Ca2+-dependent conformational changes (measured by fluorescence), and characteristics of contraction in skinned muscle fiberssurrogate

This study provides direct evidence that Ca2+-binding site II in cardiac troponin C is essential for triggering muscle contraction, while site I functions to modify contraction characteristics.

Cite This Study

Putkey et al. (1989) studied this question.

synapsesocial.com/papers/6a1c0bf94ebd09f3dfa96020 https://doi.org/10.1016/s0021-9258(18)63868-2

Also Consider

Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

View Full Paper

Ask AI

Bookmark