Phosphatase-free Crystalline Micrococcal Nuclease

This report relates to the preceding paper by Taniuchi and Anfinsen. Micrococcal nuclease from the strain Foggi has been purified from a commercially available source (Worthington). The enzyme was crystallized from an ammonium sulfate solution. The over-all purification was approximately 500-fold with an over-all yield of about 16%. The enzyme is virtually devoid of acid and alkaline phosphatases.