What question did this study set out to answer?

To identify the streptococcal surface receptor responsible for high-affinity fibronectin binding and evaluate its role in host epithelial adherence.

July 1, 1992Proceedings of the National Academy of SciencesOpen Access

Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes.

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Authors

EHEmanuel HanskiHebrew University of Jerusalem MCMichael G. CaparonInstitute for Women's Policy Research

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Implication

Experimental study reveals that protein F mediates fibronectin binding in Streptococcus pyogenes, indicating its key role in epithelial cell adherence.

Key Points

To identify the streptococcal surface receptor responsible for high-affinity fibronectin binding and evaluate its role in host epithelial adherence.
Cloned and expressed the protein F gene (prtF) in Escherichia coli to test fibronectin binding capacity.
Inactivated prtF via insertional mutagenesis and introduced the defective allele into the Streptococcus pyogenes chromosome through homologous recombination.
Assessed fibronectin-binding affinity and adherence to respiratory epithelial cells in wild-type, mutant, and plasmid-complemented strains.
Expression of cloned prtF conferred functional, high-affinity fibronectin-binding capability to E. coli.
Chromosomal disruption of prtF in S. pyogenes abolished protein F production and eliminated fibronectin-binding activity, which was successfully restored by plasmid complementation.
Mutant S. pyogenes strains lacking protein F exhibited a substantially lower capacity to adhere to respiratory epithelial cells compared to the wild type.

Cite This Study

Hanski et al. (1992) studied this question.

synapsesocial.com/papers/6a1c1f9c1567d2fc4d5fa0c2 https://doi.org/10.1073/pnas.89.13.6172

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Implication

Experimental study reveals that protein F mediates fibronectin binding in Streptococcus pyogenes, indicating its key role in epithelial cell adherence.

Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes.

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Implication

Key Points

Cite This Study

Protein F, a fibronectin-binding protein, is an adhesin of the group A streptococcus Streptococcus pyogenes.

Discussion

Member takes

Implication

Key Points

Cite This Study

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