Key result
Phosphorylation of both phospholamban and cardiac troponin I contributes to the increased rate of cardiac relaxation during beta-adrenergic stimulation with isoproterenol in mice.
Why the study?
Does beta-adrenergic stimulation with isoproterenol alter relaxation kinetics in mice lacking phospholamban and/or cardiac troponin I?
Population
Mice generated by crossbreeding mice expressing slow skeletal troponin I with phospholamban knockout mice…
Comparison
Perfusion/stimulation with isoproterenol (ISO) vs Absence of isoproterenol (ISO)
Design
Preclinical
Authors
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Supports dual phosphorylation requirement for lusitropy in mice; hypothesis-generating with clinical translation untested.
Does beta-adrenergic stimulation with isoproterenol alter relaxation kinetics in mice lacking phospholamban and/or cardiac troponin I?
Phosphorylation of both phospholamban and cardiac troponin I is required for the increased rate of cardiac relaxation observed during beta-adrenergic stimulation.
Wolska et al. (2002) studied Cardiac relaxation during beta-adrenergic stimulation. Isoproterenol (ISO) perfusion vs. Absence of ISO / different genetic mouse lines was evaluated on Relaxation rate and extent of shortening. Phosphorylation of both phospholamban and cardiac troponin I contributes to the increased rate of cardiac relaxation during beta-adrenergic stimulation with isoproterenol in mice.
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