Key result
A protocol describing three different methods to enrich fibroblasts and myofibroblasts from uninjured and injured mouse hearts was presented to enable the study of their roles in fibrosis modulation.
Why the study?
Fibroblast research has been hindered by a lack of universally acceptable markers to identify quiescent and activated fibroblasts, and their heterogeneity makes isolation and characterization difficult.
Provides a standardized protocol for isolating and characterizing cardiac fibroblasts and myofibroblasts from mouse models to facilitate research on cardiac fibrosis.
Enables standardized preclinical cardiac fibroblast isolation in mice; leaves open translation to human fibrosis therapies.
Cardiac fibrosis in response to injury is a physiological response to wound healing. Efforts have been made to study and target fibroblast subtypes that mitigate fibrosis. However, fibroblast research has been hindered due to the lack of universally acceptable fibroblast markers to identify quiescent as well as activated fibroblasts. Fibroblasts are a heterogenous cell population, making them difficult to isolate and characterize. The presented protocol describes three different methods to enrich fibroblasts and myofibroblasts from uninjured and injured mouse hearts. Using a standard and reliable protocol to isolate fibroblasts will enable the study of their roles in homeostasis as well as fibrosis modulation.
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Melzer et al. (2020) studied Cardiac fibrosis. Three different methods to enrich fibroblasts and myofibroblasts was evaluated. A protocol describing three different methods to enrich fibroblasts and myofibroblasts from uninjured and injured mouse hearts was presented to enable the study of their roles in fibrosis modulation.
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