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SummaryA high resolution technic is described for localization of intracellular sites of erythrocyte esterases. Blood smears are fixed in 1% osmium tetroxide in dimethylformamide at −15°C and then incubated in medium containing alpha naphthyl acetate and “hexaazotized” pararosanilin. Alpha naphthol released by enzymatic hydrolysis of the substrate ester couples with “hexaazonium”salt to form a colored, insoluble, amorphous deposit at sites of esterase activity.
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B. J. Davis (1959) studied this question.
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