What question did this study set out to answer?

To determine whether intermediate gene expression from the araBAD promoter at subsaturating inducer concentrations reflects uniform intermediate activity across single cells or a heterogeneous population.

July 22, 1997Proceedings of the National Academy of SciencesOpen Access

Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations

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Authors

DSDeborah A. Siegele JHJames C. Hu

Discussion

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Overview

Single-cell analysis reveals bimodal gene expression from the araBAD promoter at low arabinose levels, indicating population averages mask all-or-none cellular induction.

Key Points

To determine whether intermediate gene expression from the araBAD promoter at subsaturating inducer concentrations reflects uniform intermediate activity across single cells or a heterogeneous population.
Constructed plasmids driving the expression of a fast-folding mutant green fluorescent protein from the araBAD promoter.
Used fluorescence microscopy to examine single-cell induction patterns and expression kinetics across bacterial cultures exposed to subsaturating arabinose concentrations.
Single-cell microscopic observation at low arabinose concentrations demonstrated a mixed population of brightly fluorescent cells and dark, uninduced cells.
Intermediate bulk expression levels reflected a population-wide average of all-or-none single-cell responses driven by an autocatalytic active transport mechanism.

Cite This Study

Siegele et al. (1997) studied this question.

synapsesocial.com/papers/6a1c96fd66d062ff2dc3fe47 https://doi.org/10.1073/pnas.94.15.8168

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July 22, 1997Proceedings of the National Academy of SciencesOpen Access

Gene expression from plasmids containing the araBAD promoter at subsaturating inducer concentrations represents mixed populations

View Full Paper

Ask AI

Bookmark

Authors

DSDeborah A. Siegele JHJames C. Hu

Discussion

Member takes

Overview

Single-cell analysis reveals bimodal gene expression from the araBAD promoter at low arabinose levels, indicating population averages mask all-or-none cellular induction.

Key Points

To determine whether intermediate gene expression from the araBAD promoter at subsaturating inducer concentrations reflects uniform intermediate activity across single cells or a heterogeneous population.
Constructed plasmids driving the expression of a fast-folding mutant green fluorescent protein from the araBAD promoter.
Used fluorescence microscopy to examine single-cell induction patterns and expression kinetics across bacterial cultures exposed to subsaturating arabinose concentrations.
Single-cell microscopic observation at low arabinose concentrations demonstrated a mixed population of brightly fluorescent cells and dark, uninduced cells.
Intermediate bulk expression levels reflected a population-wide average of all-or-none single-cell responses driven by an autocatalytic active transport mechanism.

Cite This Study

Siegele et al. (1997) studied this question.

synapsesocial.com/papers/6a1c96fd66d062ff2dc3fe47 https://doi.org/10.1073/pnas.94.15.8168

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