What question did this study set out to answer?

To determine whether monocyte chemoattractant protein-3 (MCP-3) acts as a functional ligand and agonist across distinct CC chemokine receptors, specifically CC CKR1 and CC CKR2B.

December 1, 1995Journal of Biological ChemistryOpen Access

Monocyte Chemoattractant Protein-3 Is a Functional Ligand for CC Chemokine Receptors 1 and 2B

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Authors

CCChristophe CombadièreCentre National de la Recherche Scientifique SASunil K. AhujaSouth Texas Veterans Health Care System JDJo Van DammeKU Leuven

Discussion

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Implication

Experimental study demonstrates that MCP-3 activates both CC CKR1 and CC CKR2B in leukocytes, indicating distinct receptor pathways mediate monocyte and eosinophil recruitment.

Key Points

To determine whether monocyte chemoattractant protein-3 (MCP-3) acts as a functional ligand and agonist across distinct CC chemokine receptors, specifically CC CKR1 and CC CKR2B.
Assessed receptor binding affinity and specificity using radiolabeled 125I-MCP-3 competition assays in transfected cell lines expressing CC CKR1 or CC CKR2B.
Measured intracellular calcium ([Ca2+]i) mobilization to determine agonistic potency and signaling efficacy across different leukocyte subtypes.
125I-MCP-3 bound directly to both CC CKR1 (Ki = 8 nM) and CC CKR2B (Ki = 7 nM); CC CKR1 binding was competed by multiple CC chemokines, whereas CC CKR2B binding was competed exclusively by MCP-3 and MCP-1.
MCP-3 acted as an agonist for CC CKR1 with a potency rank order of MIP-1α > MCP-3 > RANTES, and exhibited equipotent agonism with MCP-1 on CC CKR2B (EC50 = 10 nM for [Ca2+]i changes).

Cite This Study

Combadière et al. (1995) studied this question.

synapsesocial.com/papers/6a1ca2053e9e446a9a85b680 https://doi.org/10.1074/jbc.270.50.29671

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Implication

Experimental study demonstrates that MCP-3 activates both CC CKR1 and CC CKR2B in leukocytes, indicating distinct receptor pathways mediate monocyte and eosinophil recruitment.

Monocyte Chemoattractant Protein-3 Is a Functional Ligand for CC Chemokine Receptors 1 and 2B

Discussion

Member takes

Implication

Key Points

Cite This Study

Monocyte Chemoattractant Protein-3 Is a Functional Ligand for CC Chemokine Receptors 1 and 2B

Discussion

Member takes

Implication

Key Points

Cite This Study

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