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In the triglycerides of palm oil (or any vegetable oil or fat), an unsaturated fatty acid (oleic or linoleic) attached to the 2‐glycerol carbon can be distinguished from one attached to the 1‐ or 3‐glycerol carbon by high (or medium) field 13 C nuclear magnetic resonance (NMR) analysis of the olefinic carbons. The chemical shift difference of the olefinic carbons in the fatty acid chain is characteristic of the chain's glycerol position and can therefore be used to identify the glycerol position of an unsaturated fatty acid in the triglyceride.
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Soon Ng (1984) studied this question.
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