What question did this study set out to answer?

To establish and validate restriction site associated DNA (RAD) markers as a rapid, low-cost platform for genome-wide polymorphism detection and high-density genotyping.

December 22, 2006Genome ResearchOpen Access

Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers

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To establish and validate restriction site associated DNA (RAD) markers as a rapid, low-cost platform for genome-wide polymorphism detection and high-density genotyping.
Generated genome-wide RAD tags using restriction enzyme cleavage to capture sequence polymorphisms at cut sites.
Tested RAD marker detection on existing fruit fly tiling path microarrays to map a recombination breakpoint.
Constructed a dedicated, low-cost RAD marker microarray to conduct bulk-segregant analysis in threespine sticklebacks.
Mapped a fruit fly recombination breakpoint at high resolution using pre-existing tiling microarrays.
Identified thousands of RAD markers in threespine stickleback, mapping a previously known locus and discovering a second region linked to lateral plate phenotype.
Demonstrated robust, high-throughput individual and bulk-segregant genotyping applicable to both model and nonmodel organisms.

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Implication

Methodological study demonstrates high-resolution genotyping using restriction site associated DNA markers in model organisms, highlighting a scalable approach for genetic mapping.

Cite This Study

Miller et al. (2006) studied this question.

synapsesocial.com/papers/6a1d1dec102421609404e0d3 https://doi.org/10.1101/gr.5681207

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Implication

Methodological study demonstrates high-resolution genotyping using restriction site associated DNA markers in model organisms, highlighting a scalable approach for genetic mapping.

Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers

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Discussion

Implication

Cite This Study

Rapid and cost-effective polymorphism identification and genotyping using restriction site associated DNA (RAD) markers

Key Points

Discussion

Implication

Cite This Study

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