Effect of p22phox depletion on sympathetic regulation of blood pressure in SHRSP: evaluation in a new congenic strain

Figures

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  Figure 1. Response to Glu microinjection in SHRSP, SHR and WKY. (a) Representative recordings of response to Glu microinjection. (b,c) Increase in SBP and in HR on Glu microinjection, respectively. Numbers of rats used in the experiments were 5 SHRSP, 5 SHR and 4 WKY in each experiment. *Significantly different when compared with the response in SHRSP. †Significantly different from the response to glutamate (G) alone. #Significantly different from the response in SHR. The Bonferroni’s post-hoc test was applied for the analyses. G: glutamate, L: losartan, T: tempol.

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  Figure 2. Response to Glu microinjection in PM0 and SP.MES. (a) Representative recordings of response to Glu microinjection. (b,c) Increase in SBP and in HR on Glu microinjection, respectively. Numbers of rats used in the experiments were; 14 PM0 and 11 SP.MES for Glu alone (G), 7 PM0 and 7 SP.MES for losartan + Glu (L+G), 8 PM0 and 5 SP.MES for tempol + Glu (T+G), and 7 PM0 and 5 SP.MES for apocynin + Glu (A+G). *Significantly different when compared with PM0. †Significantly different when compared with the response to Glu alone. The Bonferroni’s post-hoc test was applied for the analyses. G: Glu, T: tempol, L: losartan, A: apocynin.

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  Figure 3. ROS production in the brainstem of SHRSP, SHR, WKY and SP.MES. ROS production in the brainstem was quantified by the lucigenin-enhanced chemiluminescence method as described in Methods. Numbers of rats used were 7 PM0, 5 SHR, 4 WKY and 5 SP.MES. *Significantly different when compared with SHRSP. †Significantly different from the baseline level [i.e., NADPH (−) and Apo (−)]. #Significantly different when compared with the level under NADPH (+).The Bonferroni’s post-hoc test was applied for the analyses. Apo; apocynin, RLU; relative light unit.

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  Figure 4. Effects of cold stress in PM0 and SP.MES. Effects of cold stress on SBP: Numbers of rats used were 5 PM0 and 3 SP.MES. (a) The time course of BP changes is shown (b) SBP at room temperature (RT) and under cold stress (CS) are summarized (c) increase in SBP under cold stress (ΔSBP). *Significantly lower than SBP in PM0. Effects of cold stress on u-NE: Numbers of rats used are 7 PM0 and 5 SP.MES. (d) u-NE at room temperature (RT) and under cold stress (CS) are summarized (e) increase in u-NE under cold stress (Δu-NE). *Significantly different from the level in PM0. RT; room temperature, CS; cold stress, u-NE; urinary norepinephrine, SBP; systolic blood pressure. (f) Effect of losartan on response to cold stress in SHRSP. Response to cold stress was examined with and without losartan infused into the lateral ventricle. Numbers of rats used were 3 in each experiment. *Significantly different from Los (−). Los: losartan. The Student’s t-test was applied in the analyses.