Nucleotide exchange, structure, and mechanical properties of filaments assembled from ATP-actin and ADP-actin.
Population
Actin monomers and filaments assembled from ATP-actin and ADP-actin
Comparison
Assembly into filaments from ATP-actin or… vs ATP-actin filaments vs ADP-actin filaments
Design
Preclinical
Authors
Discussion
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Member takes
Implication
Challenges prior actin stiffening hypothesis; leaves open nucleotide effects on cytoskeletal dynamics in cardiovascular cells.
Structured PICO
P
Population
Actin monomers and filaments assembled from ATP-actin and ADP-actin
I
Intervention
Assembly into filaments from ATP-actin or ADP-actin
C
Comparator
ATP-actin filaments vs ADP-actin filaments
O
Outcome
Nucleotide exchange rate, structure by electron microscopy, and dynamic elasticity and viscositysurrogate
ATP hydrolysis within actin filaments does not appear to stiffen the polymer or alter its structure and affinity for nucleotides, challenging previous findings.
Cite This Study
Pollard et al. (1992) studied this question.