What question did this study set out to answer?

Determine whether Alzheimer's neurofibrillary tangles share cross-reactive antigenic determinants with normal mammalian neurofilament proteins.

January 1, 1982Journal of NeuroscienceOpen Access

Immunostaining of neurofibrillary tangles in Alzheimer's senile dementia with a neurofilament antiserum

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Determine whether Alzheimer's neurofibrillary tangles share cross-reactive antigenic determinants with normal mammalian neurofilament proteins.
Performed immunofluorescence and peroxidase-antiperoxidase staining on cryostat sections of hippocampus, frontal cortex, and cerebellum from two patients with senile dementia (N=2) using anti-chicken brain neurofilament antisera.
Tested cross-reactivity and binding specificity via immunoabsorption with bovine brain intermediate filament fractions and microtubules, and characterized target polypeptides by SDS-PAGE.
Neurofilament antisera selectively stained neurofibrillary tangles in hippocampal and cortical sections, binding the 200,000-, 150,000-, and 70,000-dalton neurofilament polypeptides on SDS-PAGE.
Tangle immunostaining was abolished by preabsorption with native antigen or bovine neurofilament preparations, but remained unaffected by bovine microtubule preparations or antisera against GFAP, vimentin, and desmin.

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Implication

Postmortem study demonstrates neurofilament antisera immunoreactivity with Alzheimer's neurofibrillary tangles, indicating tangles share antigenic properties with native neurofilaments.

Cite This Study

Dahl et al. (1982) studied this question.

synapsesocial.com/papers/6a1d601f73c56dd1bd2f96f4 https://doi.org/10.1523/jneurosci.02-01-00113.1982

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Implication

Postmortem study demonstrates neurofilament antisera immunoreactivity with Alzheimer's neurofibrillary tangles, indicating tangles share antigenic properties with native neurofilaments.

Immunostaining of neurofibrillary tangles in Alzheimer's senile dementia with a neurofilament antiserum

Key Points

Discussion

Implication

Cite This Study

Immunostaining of neurofibrillary tangles in Alzheimer's senile dementia with a neurofilament antiserum

Key Points

Discussion

Implication

Cite This Study

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