What question did this study set out to answer?

To evaluate how low oxygen concentrations influence the survival, proliferation, and repopulation capacity of hematopoietic stem and progenitor cells in bone marrow cultures.

October 1, 1993BloodOpen Access

The role of hypoxia in the maintenance of hematopoietic stem cells

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To evaluate how low oxygen concentrations influence the survival, proliferation, and repopulation capacity of hematopoietic stem and progenitor cells in bone marrow cultures.
Incubated bone marrow liquid cultures across oxygen concentrations from 0% to 18% (ambient air) for up to 6 days.
Assayed cells from day-5 severely hypoxic (1% oxygen) and control cultures for day-7 in vitro colonies, day-14 spleen colonies, radioprotective ability, and marrow repopulation capacity.
Total cell yield was directly proportional to oxygen levels, but cells from 1% hypoxia showed enhanced day-14 spleen colony formation, greater radioprotection, and increased marrow repopulation efficiency compared to air-incubated controls.
While ambient air favored expansion of late day-7 progenitors, hypoxic conditions preferentially preserved primitive progenitors sustaining marrow repopulating ability (MRACFU-NM) at the top of the hematopoietic hierarchy.

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Implication

Experimental study demonstrates hypoxia maintains primitive marrow repopulating cells in bone marrow cultures, suggesting low oxygen preserves stemness within the hematopoietic hierarchy.

Cite This Study

Cipolleschi et al. (1993) studied this question.

synapsesocial.com/papers/6a1d6da95b7fddc35205457e https://doi.org/10.1182/blood.v82.7.2031.2031

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Implication

Experimental study demonstrates hypoxia maintains primitive marrow repopulating cells in bone marrow cultures, suggesting low oxygen preserves stemness within the hematopoietic hierarchy.

The role of hypoxia in the maintenance of hematopoietic stem cells

Key Points

Discussion

Implication

Cite This Study

The role of hypoxia in the maintenance of hematopoietic stem cells

Key Points

Discussion

Implication

Cite This Study

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