What question did this study set out to answer?

Determine the thermodynamic influence of diverse solvent mixtures and salt concentrations on the stability of the model RNA double helix A7U7p.

April 9, 1985Biochemistry

Solvent effects on the stability of A7U7p

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Authors

DHDavid R. HickeyUniversity of Rochester DTDouglas H. TurnerAER Group (United States)

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Implication

In vitro thermodynamic analysis reveals solvent-dependent stability profiles in A7U7p duplexes, suggesting hydrophobic bonding does not drive RNA helix formation.

Key Points

Determine the thermodynamic influence of diverse solvent mixtures and salt concentrations on the stability of the model RNA double helix A7U7p.
Measured double-helix formation thermodynamics spectrophotometrically for A7U7 in aqueous 1 M NaCl and for A7U7p across varying sodium ion concentrations (0.06 to 0.55 M Na+) and solvent mixtures.
Evaluated stability across 10 mol % nonaqueous solvent systems and correlated results with calorimetric data and solvent surface tension.
Between 0.06 and 0.55 M Na+, the thermal melting slope dTm/d(log [Na+]) for A7U7p was 17.4 °C.
The A7U7p duplex demonstrated peak stability in 100% water at 1 M NaCl, with 10 mol % co-solvents decreasing stability in the order: ethylene glycol > glycerol > ethanol > 2-propanol > dimethyl sulfoxide > 1-propanol > formamide > N,N-dimethylformamide > urea > dioxane.
Comparison with protein thermodynamic models and solvent surface tension revealed that neither hydrophobic interactions nor cavity formation energy drive A7U7p duplex stability.

Cite This Study

Hickey et al. (1985) studied this question.

synapsesocial.com/papers/6a1d7a0573c56dd1bd2fba86 https://doi.org/10.1021/bi00329a042

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Implication

In vitro thermodynamic analysis reveals solvent-dependent stability profiles in A7U7p duplexes, suggesting hydrophobic bonding does not drive RNA helix formation.

Solvent effects on the stability of A7U7p

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Implication

Key Points

Cite This Study

Solvent effects on the stability of A7U7p

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Implication

Key Points

Cite This Study

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